Dr. Mahadev Kalyankar

PhD
Associate Professor,
School of Medical Sciences,
University of Hyderabad
Email: mahadev_kalyankar@yahoo.co.in; mkmd@uohyd.ernet.in
Phone: +91-040-23135478

Dr. Kalyankar have been working in the field of Diabetes, Obesity and Metabolic Disorders since 18 years. Through this period, he has spearheaded to find some of the new exciting research outcomes that opened up new avenues in the field of diabetes, insulin signaling and metabolic disorders. During this period of his research career, he has published 29 research articles in peer reviewed journals such as Diabetes, Molecular and Cellular Biology, Journal of Biological Chemistry, FASEB Journal, Experimental Cell Research, Journal of Clinical Investigation etc. All these publications have been cited nearly 5000 times so far.

Highlights of my accomplishments in the field:

  • Discovered that Perilipin, a lipid droplet protein can distinguish between simple Liver Steatosis (NAFLD) and Non-Acoholic Steatohepatitis (NASH) in Adults and Children.
  • Discovered that insulin generated oxidant signal enhances insulin sensitivity in Type 2 diabetes and other clinical states of insulin resistance
  • Identified for the first time that insulin stimulated ROS reversibly inhibits protein tyrosine phosphatases, PTP1B, and established a new technique for live cell imaging of ROS. (Mahadev et al., 2001a, JBC. 276: 21938-21942. (Cited so far 532 times); Mahadev et al., 2001b, JBC. 276: 48662-48669. (Cited so far 311 times).
  • Discovered and characterized a novel protein NADPH Oxidase 4 in insulin sensitive cells that opened up new avenues for enhancing insulin sensitivity in Type 2 diabetes and clinical states of insulin resistance. (Mahadev et al, 2004, MCB, 24: 1844-1854. (Cited so far 522 times).
  • Demonstrated that restoration of cytoskeletal protein tropomyosin-1 expression in breast cancer cells stabilizes adherent junctions and that contributes to anti-oncogenic properties of this protein. (Mahadev et al., 2002, Experimental Cell Research, 279: 40-51

Research areas

Metabolic Disorders, NAFLD / NASH, Insulin Signaling Diabetes and Obesity


Selected Publications

S. No.AuthorsTitleJournalVolPageYear
1 Carr RM, Dhir R, Mahadev K, Comerford M, Chalasani NP, Ahima RS. Perilipin Staining Distinguishes Between Steatosis and Nonalcoholic Steatohepatitis in Adults and Children. Clin Gastroenterol Hepatol. 16 30567 2017
2Mandal CC, Ganapathy S, Gorin Y, Mahadev K, Block K, Abboud HE, Ghosh- Choudhury G, and Ghosh- Choudhury N. Nox4-Derived Physiologic Reactive Oxygen Species Mediate Bmp-2-Induced Bmp-2 Gene Transcription And Osteoblast Differentiation Biochem J. 15 393 2011
3Li J, Wang JJ, Yu Q, Chen K, Mahadev K, Zhang SX Inhibition of reactive oxygen species by lovastatin down- regulates VEGF expression and ameliorates blood- retinal barrier breakdown in db/db mice: role of NADPH oxidase 4. Diabetes 59 1528 2010
4 Mahadev K*, Wu X, Donnelly S, Ouedraogo R,
Eckhart AD, Goldstein BJ.
Adiponectin inhibits vascular endothelial growth factor-induced migration of human coronary artery endothelial cells. Cardiovascular Research. 78 376 2008
5 Xu SQ* , *Mahadev K , Wu X, Fuchsel L, Donnelly S, Ouedraogo R, Goldstein BJ. Adiponectin protects against angiotensin II or tumor necrosis factor – alpha induced endothelial cell monolayer hyperpermeability: Role of cAMP/PKA signaling. Arterioscler Thromb Vasc Biol. 28 899 2008
6 Nakamura Y, Patrushev N, Inomata H, Mehta D, Kim HW, Urao N, Razvi M, Kini V, Mahadev K, Goldstein BJ, Mckinney R, Fukai T, Ushio- Fukai M. Role of Protein Tyrosine Phosphatase 1B in VEGF Signaling and Cell-Cell Adhesions in Endothelial Cells. Circulation Res. 102 1182 2008
7 Wu X, Mahadev K, Fuchsel L, Ouedraogo R, Xu SQ, Goldstein BJ. Adiponectin suppresses I{kappa}B kinase activation induced by tumor necrosis factor-{alpha} or high glucose in endothelial cells: role of cAMP and AMP kinase signaling. Am J Physiol Endocrinol Metab. 200 1836 2007
8 Datla SR, Peshavariya H, Dusting GJ, Mahadev K, Goldstein BJ, Jiang F. Important role of Nox4 type NADPH oxidase in angiogenic responses in human microvascular endothelial cells in vitro. Arterioscler Thromb Vasc Biol. 27 2319 2007
9 Ouedraogo R, Gong Y, Berzins B, Wu X, Mahadev K, Hough K, Chan L, Goldstein BJ, Scalia R. Adiponectin deficiency increases leukocyte- endothelium interactions via upregulation of endothelial cell adhesion molecules in vivo. Journal of Clinical Investigation.117 1718 2007
10 Ouedraogo R, Wu X, Xu SQ, Fuchsel L, Motoshima H, Mahadev K, Hough K, Scalia R, Goldstein BJ. Adiponectin suppression of high-glucose- induced reactive oxygen species in vascular endothelial cells: evidence for involvement of a cAMP signaling pathway. Diabetes 55 1840 2006
11 Wu X., Zhu L, Mahadev K, Motoshima H, Zilbering A, Yao J, and Goldstein BJ. Hyperglycemia Potentiates H2O2 Production in Adipocytes and Enhances Insulin Signal Transduction: Potential Role for Oxidative Inhibition of Thiol Sensitive Protein- Tyrosine Phosphatases. Antioxid Redox Signal. 7 526 2005
12 Hu T, Ramachandra Rao S, Siva S, Valancius C, Zhu Y, Mahadev K, Toh I, Goldstein BJ, Woolkalis M, Sharma K. Reactive Oxygen Species Production via NADPH oxidase mediates TGF-beta Induced Cytoskeletal Alterations in Endothelial Cell. Am J Physiol Renal Physiol. 289 8162005
13Goldstein BJ, Mahadev K, and Wu X. Redox Paradox: Insulin Action is Facilitated by Insulin-Stimulated Reactive Oxygen Species with Multiple Potential Signaling Targets. Diabetes 54 311 2005
14 Goldstein BJ, Mahadev K, and Wu X, Zhu L, Motoshima H. Role of Insulin- Induced Reactive Oxygen Species in the Insulin Signaling Pathway. Antioxid Redox Signal. 7 1021 2005
15 Mahadev K, Motoshima H, Wu X, Cheng G, Lambeth JD, Goldstein BJ. The NADPH Oxidase Homolog Nox4 Modulates Insulin-Stimulated Generation of H2O2 and Plays an Integral Role in Insulin Signal Transduction. Molecular and Cellular Biology 2004, 24: 1844- 1854. 24 1844 2004
16 Mahadev K, Wu X., Motoshima H, and Goldstein BJ. Integration of Multiple down- stream Signals Determines the Net Effect of Insulin on MAPK vs. PI 3’- Kinase. Activation: Potential Role of Insulin- Stimulated H2O2. Cell Signalling 16 323 2004
17 Motoshima H, Wu X, Mahadev K, Goldstein BJ. Globular Adiponectin inhibits oxidized LDL- induced superoxide generation, Cell Proliferation and oxidative modification of native LDL in bovine aortic endothelial cells. Biochemical and Biophysical Research Communications 315 264 2004
18 Wu X, Motoshima H, Mahadev K, Stalker TJ, Scalia R, Goldstein BJ. Involvement of AMP- activated protein kinase in glucose uptake stimulated by adiponectin in primary rat adipocytes. Diabetes 52 1355 2003
19 Wu X, Hardy VE, Joseph JI, Jabbour S, Mahadev K, Zhu L, Goldstein BJ. Protein-tyrosine phosphatase activity in human adipocytes is strongly correlated with insulin- stimulated glucose uptake and is a target of insulin- induced oxidative inhibition. Metabolism. 52 705 2003
20 Mahadev K., Raval G, Bharadwaj S, Willingham MC, Lange EM, Vonderhaar B, Salomon D, Prasad GL. Suppression of the transformed phenotype of breast cancer by tropomyosin-1. Experimental Cell Research 279 40 2002
21 Mahadev K, Wu X, Zilbering A, Zhu L, Lawrence T, Goldstein BJ. Hydrogen peroxide generated during cellular insulin stimulation is integral to activation of the insulin signaling cascade in 3T3-L1 adipocytes. Journal of Biological Chemistry 276 48662 2001
22 Mahadev K, Zilbering A, Zhu L, Goldstein BJ. Insulin-stimulated generation of hydrogen peroxide reversibly inhibits PTP1B and enhances the early insulin action cascade. Journal of Biological Chemistry 276 21938 2001
23 Zhu L, Zilbering A, Wu X, Mahadev K, Joseph JI, Jabbour S, Deeb W, Goldstein B J. Use of an anaerobic environment to preserve the endogenous activity of protein- tyrosine phosphatases isolated from intact cells. FASEB J. 15 1637 2001
24 Mahadev K, Chetty SC, Vemuri MC. Effect of prenatal and postnatal ethanol exposure onCa2+/calmodulin- dependent protein kinase II in rat cerebral cortex. Alcohol. 23 183 2001
25 Mahadev K and Vemuri MC. Effect of pre and postnatal ethanol exposure on protein tyrosine kinase and its endogenous substrate proteins in rat cerebral cortex. Alcohol. 17 223 1999
26 Mahadev K and Vemuri, MC. Selective changes in Protein kinase C isoforms and phosphorylation of endogenous substrate proteins in rat cerebral cortex during pre- and postnatal ethanol exposure. Archives of Biochemistry & Biophysics. 356 249 1998
27 Mahadev K and Vemuri MC. Effect of ethanol on chromatin and non-histone nuclear proteins in rat brain. Neurochemical Research 23 1179 1998
28 Mahadev K and Vemuri MC. Ethanol induced changes in hepatic chromatin and non- histone nuclear protein composition in rat. Alcohol. 15 207 1998

Research Projects

  1. Training grant in Insulin signal transduction by National Institute of Health (completed in 2006).
  2. NADPH Oxidase Homolog Nox4 and Its Interacting Proteins in Insulin Signaling by the Department of Biotechnology (completed in 2017).

Invited talks

  1. Discovery of Novel Signaling Pathways by Insulin and Adiponectin. University of Hyderabad, India 2008.
  2. The NADPH Oxidase Homolog Nox4 Modulates Insulin-Stimulated Generation of H2O2 and Plays an Integral Role in Insulin Signal Transduction. Thomas Jefferson University, Philadelphia. 2005.